RP-HPLC (STABILITY-INDICATING) BASED ASSAY METHOD FOR THE SIMULTANEOUS ESTIMATION OF DORAVIRINE, TENOFOVIR DISOPROXIL FUMARATE AND LAMIVUDINE

Objective: In this study, a RP-HPLC (stability-indicating) based assay method for the estimation of doravirine (DRV), tenofovir disoproxil fumarate (TFF) and lamivudine (LMV) simultaneously in tablets was described.
 Methods: The simultaneous analysis DRV, TFF LMV done with HPLC system (Agilent 1100 series) Luna Phenomenex C18 (250 mm × 4.6 5 μ) column isocratic mobile phase (35% volume ratio methanol 65% 20 mmol ammonium formate, pH 5). Validation on sensitivity, linearity, accuracy, selectivity, precision, robustness specificity.
 Results: calibration curves were linear through range 25-200 µg/ml DRV 75-600 LMV. percent relative standard deviation intraday variation/precision, interday intermediate precision/ruggedness lower than 2%. recovery (99.09-99.76%), (99.10-99.41%) (98.65-99.28%) confirmed good accuracy. stability LMV, 0.1N NaOH, 3% peroxide, 0.1 N HCl, UV light dry heat 60 °C determined.
 Conclusion: results have allowed to be implemented quantify TFF,